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Human TNFSF11 / RANKL / CD254 Protein, His tag  pdf  pdf  pdf


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RAL-H5240-50ug
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Synonym

RANKL,CD254,TRANCE,OPGL,ODF

Source

Human TNFSF11, His Tag (RAL-H5240) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Predicted N-terminus: His

Molecular Characterization

Poly-his
TNFSF11(Gly 64 - Asp 245)AAC51762.1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 23.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human TNFSF11, His Tag (Cat. No. RAL-H5240) SDS-PAGE gel

Human TNFSF11, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human TNFSF11, His Tag (Cat. No. RAL-H5240) ELISA bioactivity

Immobilized Human TNFRSF11A at 5 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (Cat. No. RAL-H5240) with a linear range of 0.2-6 ng/mL (QC tested).

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Background

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

References

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