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Human MS4A1 / CD20 Protein, Fc Tag  pdf  pdf  pdf


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CD0-H5268-100ug
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Synonym

MS4A1,CD20,MS4A-1

Source

Human MS4A1, Fc Tag (CD0-H5268) is expressed from human 293 cells (HEK293). It contains AA Ile 141 - Ser 188 (Accession # P11836-1).

Predicted N-terminus: Pro

Molecular Characterization

Fc(Pro 100 - Lys 330)P01857
MS4A1(Ile 141 - Ser 188)P11836-1

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 33.4 kDa. The protein migrates as 42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human MS4A1, Fc Tag (Cat. No. CD0-H5268) SDS-PAGE gel

Human MS4A1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Human MS4A1, Fc Tag (Cat. No. CD0-H5268) ELISA bioactivity

Immobilized Human MS4A1, Fc Tag (Cat. No. CD0-H5268) at 2 μg/mL (100 μL/well) can bind Rituximab with a linear range of 0.156-5 μg/mL (QC tested).

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Background

B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. The protein has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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