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Biotinylated Mouse VEGF120 Protein, His Tag & Avi Tag (Avitag™)  pdf  pdf  pdf


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VE0-M82Q2-25ug
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Synonym

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

Source

Biotinylated Mouse VEGF120, His Tag (VE0-M82Q2) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # Q00731-3).

Predicted N-terminus: His

Molecular Characterization

Poly-his
Avi
VEGF120(Ala 27 - Arg 146)Q00731-3

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 17.3 kDa. The protein migrates as 22-24 kDa and 24-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Mouse VEGF120, His Tag (Cat. No. VE0-M82Q2) SDS-PAGE gel

Biotinylated Mouse VEGF120, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Biotinylated Mouse VEGF120, His Tag (Cat. No. VE0-M82Q2) ELISA bioactivity

Immobilized VEGFR1/R2 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF120, His Tag (Cat. No. VE0-M82Q2) with a linear range of 0.06-2 ng/mL (QC tested).

Request for protocol

Biotinylated Mouse VEGF120, His Tag (Cat. No. VE0-M82Q2) ELISA bioactivity

Immobilized Avastin at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF120, His Tag (Cat. No. VE0-M82Q2) with a linear range of 0.125-2 μg/mL (Routinely tested).

Request for protocol

 

Background

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

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