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Your Position: Home > Recombinant Proteins > Biotinylated Human IL-17 RA / CD217 Protein, His Tag, Avi Tag

Biotinylated Human IL-17 RA / CD217 Protein, His Tag & Avi Tag (Avitag™)

  • Synonym
    CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R
  • Source
    Biotinylated Human IL-17 RA, His Tag (ILR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).
    Predicted N-terminus: Leu 33
  • Molecular Characterization
    IL-17 RA(Leu 33 - Trp 320) Q96F46-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 37.2 kDa. The protein migrates as 55-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-17 RA, His Tag (Cat. No. ILR-H82E5) SDS-PAGE gel

Biotinylated Human IL-17 RA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human IL-17 RA, His TagBiotinylated Human IL-17 RA, His Tag (Cat. No. ILR-H82E5) ELISA bioactivity

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, His Tag, Avi Tag (Cat. No. ILR-H82E5) with a linear range of 0.2-8 ng/mL (QC tested).

  • Background
    Interleukin-7 receptor subunit alpha (IL-7 RA or IL7Ra) is also known as cluster of differentiation 127 (CD127). IL-7 RA is a type I cytokine receptor and is a subunit of the functional Interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. IL-7 RA has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V(D)J recombination[citation needed]. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $1380.00

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