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Your Position: Home > Protein > PCSK9 > PC9-H5223

Human PCSK9 Protein, His Tag (MALS Verified)

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  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Human PCSK9, His Tag(PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 ).
    Predicted N-terminus: Gln 31 & Ser 153
  • Molecular Characterization
    PCSK9 Structure

    This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 24 months under sterile conditions after reconstitution.
SDS-PAGE
PCSK9 SDS-PAGE

Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
PCSK9 MALS images

The purity of Human PCSK9, His Tag (Cat. No. PC9-H5223) is more than 90% and the molecular weight of this protein is around 70-80 kDa verified by SEC-MALS.

Bioactivity-ELISA
 PCSK9 ELISA

Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 5 μg/mL (100 μL/well) can bind Human LDL R, Fc Tag (Cat. No. LDR-H5254) with a linear range of 10-156 ng/mL (QC tested).

 PCSK9 ELISA

Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 2 μg/mL (100 μL/well) on an Nickel Coated plate can bind Human PCSK9 Mab (RN316), Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

 PCSK9 ELISA

Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 1 μg/mL (100 μL/well) on Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) (Cat. No. HIS-AY63) precoated (0.1 μg/well) plate can bind Evolocumab with a linear range of 0.1-4 ng/mL (Routinely tested).

Bioactivity-BLI
 PCSK9 BLI

Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 2.17 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 PCSK9 BLI

Loaded PCSK9 Mab, Human IgG1 on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 88.6 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • Clinical and Translational Updates

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