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ActiveMax® Recombinant Human TNF-alpha (HPLC-verified)  pdf  pdf  pdf


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TNA-H4211-100ug
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Synonym

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

Source

ActiveMax® Human TNF-alpha (HPLC-verified) (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Predicted N-terminus: Val 77

Molecular Characterization

TNF-alpha(Val 77 - Leu 233)NP_000585.2

This protein carries no "tag".

The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

A SEC-HPLC analysis showing 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.

Endotoxin

Less than 0.1 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 6 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H4211) SDS-PAGE gel

ActiveMax® Human TNF-alpha (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

ActiveMax® Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H4211) HPLC images

A SEC-HPLC analysis showing over 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.

 

Bioactivity

ActiveMax® Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha (Cat. No. TNA-H4211) at 5 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.04-0.6 ng/mL (QC tested).

Request for protocol

ActiveMax® Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).

Request for protocol

Human EGF R, His Tag (Cat. No. EGR-TNA-H4211) ELISA bioactivity

Immobilized Humira on CM5 Chip via anti-human Fc IgG, can bind Human TNF-alpha Protein (Cat.No. TNA-H4211) with an affinity constant of 0.114nM as determined in SPR assay (Biacore T200) ( Routinely tested).

Request for protocol

 

Background

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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