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Your Position: Home > Recombinant Proteins > Human TNF-alpha Protein,HEK293 expressed,ActiveMax, low endotoxin

ActiveMax® Recombinant Human TNF-alpha (HPLC-verified), low endotoxin

  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    Human TNF-alpha, Tag Free (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375).
    Predicted N-terminus: Val 77
  • Molecular Characterization
    TNF-alpha(Val 77 - Leu 233) P01375

    This protein carries no "tag".

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa and 18 kDa under reducing (R) condition (SDS-PAGE).

    A SEC-HPLC analysis showing 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active  trimers with a molecular mass of 54 kDa.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) SDS-PAGE gel

Human TNF-alpha, Tag Free on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human TNF-alpha, Tag Free (Cat. No. ) HPLC images

A SEC-HPLC analysis showing over 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.

Bioactivity
Human TNF-alpha, Tag FreeHuman TNF-alpha, Tag Free (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).

Human TNF-alpha, Tag FreeHuman TNF-alpha, Tag Free (Cat. No. TNA-H4211) ELISA bioactivity

Immobilized Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Humira on CM5 Chip via anti-human Fc IgG, can bind Human TNF-alpha Protein (Cat.No. TNA-H4211) with an affinity constant of 0.114nM as determined in SPR assay (Biacore T200) ( Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Recombinant Human TNF-alpha (Cat.No.  TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D.  The EC50 for this effect is 0.007-0.014ng/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Neutralization assay shows that the cytotoxicity effect of TNF-alpha (Cat.No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1ng/mL. The IC50 is 29ng/mL (Routinely tested).

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