Browse Products By Molecule:

Products --> Cytokines & Growth Factors

Human Transferrin Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
TRN-H4229-200ug
    $310.00
TRN-H4229-1mg
    $1200.00
In-stock
Place Purchase Orders


Synonym

Transferrin,TF,DKFZp781D0156,PRO1557,PRO2086

Source

Human Transferrin, His Tag (TRN-H4229) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 698 (Accession # AAH59367).

Predicted N-terminus: Val 20

Molecular Characterization

Transferrin(Val 20 - Pro 698)AAH59367
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 76 kDa. The protein migrates as 74 kDa on a SDS-PAGE gel under reducing (R) condition.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Transferrin, His Tag (Catalog # TRN-H4229) SDS-PAGE gel

Human Transferrin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human Transferrin, His Tag (Catalog # TRN-H4229) ELISA bioactivity

Immobilized Human Transferrin, His Tag (Cat# TRN-H4229) at 10 μg/mL (100 µL/well),can bind Biotinylated Human Transferrin R (Cat# TFR-H8243) with a linear range of 2-200 ng/mL (QC tested).

Request for protocol

 

Background

Transferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin”. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule . Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria.Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.