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ActiveMax® Recombinant Human SCF /KITLG  pdf  pdf  pdf


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SCF-H4212-20ug
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SCF-H4212-50ug
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SCF-H4212-1mg
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Synonym

KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

Source

ActiveMax® Human SCF (SCF-H4212) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - His 214 (Accession # AAH69797).

Predicted N-terminus: Glu 26

Molecular Characterization

SCF(Glu 26 - His 214)AAH69797

This protein carries no "tag".

The protein has a calculated MW of 21 kDa. The protein migrates as 30-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human SCF (Cat. No. SCF-H4212) SDS-PAGE gel

ActiveMax® Human SCF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity

ActiveMax® Human SCF (Cat. No. SCF-H4212) ELISA bioactivity

(1) Immobilized ActiveMax® Human SCF (Cat. No. SCF-H4212) at 3 μg/mL (100 μl/well) can bind Human CD117, Fc Tag (Cat. No. CD7-H5255) with a linear range of 0.1-1 ng/mL (QC tested).

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(2) Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The ED50 <5 ng/mL, corresponding to a specific activity of >200000 Unit/mg (Routinely tested).

 

Background

Stem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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