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ActiveMax® Recombinant Human SCF /KITLG  pdf  pdf  pdf


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Synonym

KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

Source

ActiveMax® Recombinant Human SCF /KITLG (ActiveMax® Human SCF) Glu 26 - His 214 (Accession # AAH69797) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

SCF (Glu 26-His 214)AAH69797

ActiveMax® Human SCF contains no "tag", and has a calculated MW of 21 kDa. The predicted N-terminus is Glu 26. The reducing (R) protein migrates as 30-42 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

ActiveMax® Recombinant Human SCF /KITLG

ActiveMax® Human SCF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity

(1) Immobilized ActiveMax® Human SCF (Cat# SCF-H4212) at 3 μg/mL (100 μl/well) can bind Human CD117, Fc Tag (Cat# CD7-H5255)with a linear range of 0.1-1 ng/mL.

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(2) Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The ED50 <5 ng/mL, corresponding to a specific activity of >2x105 Unit/mg.

 

 
 

Background

Stem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.

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References