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Human IgG1 Fc Protein, Tag Free  pdf  pdf  pdf


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FCC-H5214-200ug
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Synonym

IgG Fc

Source

Human IgG1 Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # AAC82527.1).

Predicted N-terminus: Glu 99

Molecular Characterization

IgG Fc(Glu 99 - Lys 330)AAC82527.1

This protein carries no "tag".

The protein has a calculated MW of 26 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) SDS-PAGE gel

Human IgG1 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) ELISA bioactivity

Immobilized Human CD64, His Tag (Cat. No. FCA-H52H2) at 10μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Cat. No. FCC-H5214) with a linear range of 7-41 ng/mL (QC tested).

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Citations

Background

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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