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Your Position: Home > Cytokines & Growth Factors > Human IGF-I Protein,N-Fc Tag,HEK293 expressed

Human IGF-I Protein, Fc Tag

  • Synonym
    IGF-I,IGF1A,somatomedin C,MGF
  • Source
    Human IGF-I, Fc Tag (IG1-H4269) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019).
    Predicted N-terminus: Gly
  • Molecular Characterization
    IGF-I(Gly 49 - Ala 118) P05019

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 35 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IGF-I, Fc Tag (Cat. No. IG1-H4269) SDS-PAGE gel

Human IGF-I, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity
Human IGF-I, Fc TagHuman IGF-I, Fc Tag (Cat. No. IG1-H4269) ELISA bioactivity

Immobilized Human IGF-I, Fc Tag (Cat. No. IG1-H4269) at 5 μg/mL (100 µl/well),can bind Human IGFBP-3, His Tag (Cat. No. IG3-H5229) with a linear range of 0.03-0.25 µg/mL (QC tested).

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