HBsAg, adw,Small S
Recombinant Hepatitis B Surface Antigen adw Subtype (rHBsAg-adw) was produced in Yeast cells(Saccharomyces cerevisiae).
rHBsAg-adw full length monomeric protein contains 226 amino acids (Met 1 - Ile 226) and the has a calculated MW of 24 kDa.The predicted N-terminus is Met.The reducing (R) protein migrates as 24 kDa in SDS-PAGE.
Less than 0.05 EU per μg by the LAL method.
>97% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 6.2 mM Phosphate, 200 mM NaCl bufer, pH 7.2. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Authors: MR Aghasadeghi et al.
Journal: Avicenna Journal of Medical Biotechnology 2014
Application: Immunization & ELISA
Amino Acid Sequence
Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. At the center of the hepatitis B virus is DNA, which contains the genes the virus uses to replicate itself. Surrounding the DNA is a protein called HBcAg (hepatitis B core antigen), which cannot be detected with blood tests. Surrounding this is HBsAg, which is actually part of the protective "envelope." This envelope surrounds the virus and protects it from attack by the body's immune system. HBsAg stands for hepatitis B surface antigen and is the surface antigen of the Hepatitis-B-Virus (HBV) S-gene. The capsid of a virus has different surface proteins from the rest of the virus. The antigen is a protein that binds specifically on one of these surface proteins. It is commonly referred to as the Australian Antigen.
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