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ActiveMax® Recombinant Human G-CSF  pdf  pdf  pdf


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Synonym

G-CSF,CSF3,C17orf33,CSF3OS,MGC45931

Source

ActiveMax® Human G-CSF (GCF-H5214) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Pro 204 (Accession # NP_757373.1).

Predicted N-terminus: Ala 31

Molecular Characterization

G-CSF(Ala 31 - Pro 204)NP_757373.1

This protein carries no "tag".

The protein has a calculated MW of 18.7 kDa. The protein migrates as 18-21 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human G-CSF (Catalog # GCF-H5214) SDS-PAGE gel

ActiveMax® Human G-CSF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

ActiveMax® Human G-CSF (Catalog # GCF-H5214) ELISA bioactivity

Immobilized Human G-CSF, Tag Free (Catalog # GCF-H5214) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, His Tag (Catalog # GCR-H82E4) with a linear range of 0.156-1.25 μg/mL.

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Background

Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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