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MabAffinity Protein L High Flow Beads,100ml  pdf  pdf  pdf


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ML-0422-03-100ml
    $2900.00
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MabAffinity Protein L High Flow Beads, with rProtein L coupled to high flow Agarose matrix, has been successfully used for a wide range of antibody and antibody fragments capture for its strong affinity to the variable region of antibody kappa light chains. Although it binds to the Fab portion of the immunoglobulin, Protein L does not interfere with the antigen-binding site of the antibody. Therefore, Protein L potentially can be used in immunoprecipitation (IP) procedures. As a good complementary choice for Protein A and Protein G, besides IgG, Protein L has been used for efficient purification of IgM, IgE, IgD, IgA and IgY. MabAffinity Protein L High Flow Beads also shows great advantages in purification of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Features:

  1. Binding specificities that complement Protein A and G media
  2. Suitable for different applications, including poly or monoclonal antibodies and antibody fragments from different species and subclasses for its broad binding specificity
  3. Purification of monoclonal antibodies from FCS supplemented media without interference of bovine immunoglobulin
  4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification:

Composition Highly cross-linked Agarose
Average particle size 90 micron
Form Slurry in 20% Ethanol
Pack size 100ml settled agarose media
(200ml 50% slurry)
Ligand rProtein L
Coupling chemistry Epoxy
Dynamic binding capacity1 > 5-10 mg human IgG /ml media
Recommended flow rate 100-300cm/hr
Chemical stability Stable in all aqueous buffers commonly used in protein L chromatography
pH working range 2-10
pH CIP range (short term) 2-11
CIP stability 8M Urea, 10mM NaOH + 1M NaCl, 0.1M acetic acid
Temperature stability1 2-40oC
Storage 20% Ethanol
Shelf life 5 years
  1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

MabAffinity Protein L High Flow Beads belongs to the MabAffinity resins family for capture of antibodies and fragments containing kappa light chains, and is available with different pack sizes for easy purification, process development and scale up. Immunoprecipitation studies with MabAffinity Protein L High Flow Beads are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

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