Browse Products By Molecule:

Products --> ActiveMax® Cytokines

ActiveMax® Recombinant Human IL-15  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
IL5-H4217-50ug
    $450.00
IL5-H4217-20ug
    $198.00
3weeks
Place Purchase Orders


Synonym

IL-15,Interleukin-15,MGC9721

Source

ActiveMax® Human IL-15 (IL5-H4217) is expressed from human 293 cells (HEK293). It contains AA Asn 49 - Ser 162 (Accession # AAH18149).

Predicted N-terminus: Asn 49

Molecular Characterization

IL-15(Asn 49 - Ser 162)AAH18149

This protein carries no "tag".

The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa on a SDS-PAGE gel under reducing (R) condition.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human IL-15 (Catalog # IL5-H4217) SDS-PAGE gel

ActiveMax® Human IL-15 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

ActiveMax® Human IL-15 (Catalog # IL5-H4217) ELISA bioactivity

Immobilized Human IL-15, Tag Free (Catalog # IL5-H4217) at 10μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (Catalog # ILB-H5253) with a linear range of 0.08-0.3 μg/mL (QC tested).

Request for protocol

 

Background

Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.