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ActiveMax® Recombinant Human IL-2  pdf  pdf  pdf


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IL2-H4216-100ug
    $195.00
IL2-H4216-1mg (500ug x 2)
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Synonym

IL2,TCGF,lymphokine,Interleukin 2

Source

ActiveMax® Human IL-2 (IL2-H4216) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568).

Predicted N-terminus: Ala 21

Molecular Characterization

IL-2(Ala 21 - Thr 153)P60568

This protein carries no "tag".

The protein has a calculated MW of 15.4 kDa. The protein migrates as 17 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human IL-2 (Cat. No. IL2-H4216) SDS-PAGE gel

ActiveMax® Human IL-2 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

ActiveMax® Human IL-2 (Cat. No. IL2-H4216) ELISA bioactivity

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4216) at 10μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) with a linear range of 0.625-5 μg/mL (Routinely tested).

Request for protocol

ActiveMax® Human IL-2 (Cat. No. IL2-H4216) ELISA bioactivity

Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 2μg/mL (100 μL/well) can bind Human IL-2, Tag Free (Cat. No. IL2-H4216) with a linear range of 0.2-1.56 ng/mL (QC tested).

Request for protocol

 

Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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