Mouse CCL6, His Tag (CC6-M5222) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ala 116 (Accession # P27784).
Predicted N-terminus: Gly 22
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 11.4 kDa. The protein migrates as 14 kDa on a SDS-PAGE gel under reducing (R) condition.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse CCL6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Chemokine (C-C motif) ligand 6 (CCL6), a member of the CC chemokine family, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GMCSF. CCL6 has only been identified in rodents, A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, where it is strongly induced upon GMCSF (Cat. # GMF-H4214) stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 (Cat. # IL4-H4218) in mouse macrophages and by cytokine IL13 (Cat. # IL3-H4210) in mouse lung. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.