Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Bio-Markers & CD Antigens

Human Siglec-3 / CD33 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
CD3-H5226-100ug
    $290.00
CD3-H5226-1mg
    $1600.00
In-stock
Place Purchase Orders


Synonym

CD33,SIGLEC3,gp67

Source

Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).

Predicted N-terminus: Asp 18

Molecular Characterization

Siglec-3(Asp 18 - His 259)AAH28152.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.6 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Siglec-3, His Tag (Cat. No. CD3-H5226) SDS-PAGE gel

Human Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human Siglec-3, His Tag (Cat. No. CD3-H5226) ELISA bioactivity

Immobilized Anti-Human CD33 MAb, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with a linear range of 0.2-1 ng/mL (QC tested).

Request for protocol

 

Background

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Related Products

Questions & Comments