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Human Neuroligin-4, Y-linked / NLGN4Y Protein  pdf  pdf  pdf


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Synonym

NLGN4Y,neuroligin 4,Y-linked,NLGN4Y1

Source

Human Neuroligin-4, Y-linked, His Tag (NL1-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 44 - Ser 676 (Accession # NP_055708.3).

Predicted N-terminus: Gln 44

Molecular Characterization

NLGN4Y(Gln 44 - Ser 676)NP_055708.3
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.23 kDa. The protein migrates as 70-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Neuroligin-4, Y-linked, His Tag (Cat. No. NL1-H5228) SDS-PAGE gel

Human Neuroligin-4, Y-linked, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Neuroligin 4, Y-linked (NLGN4Y) a type I membrane protein that belongs to the family of neuroligins, which are cell adhesion molecules present at the postsynaptic side of the synapse, and may be essential for the formation of functional synapses. Alternatively spliced transcript variants have been found for NLGN4Y gene. NLGN4Y was expressed in fetal and adult brain, prostate, testis, pancreas. NLGN4Y is homologous with its X-linked homolog, NLGN4X.

References

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