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Human Olfactomedin-4 / OLFM4 Protein  pdf  pdf  pdf


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OL4-H5221-50ug
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Synonym

OLFM4,GC1,GW112,Olfactomedin-4,KIAA4294,OLM4,OlfD,UNQ362,hGC-1,hOLfD

Source

Human Olfactomedin-4, His Tag (OL4-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Gln 510 (Accession # NP_006409).

Predicted N-terminus: Asp 21

Molecular Characterization

OLFM4(Asp 21 - Gln 510)NP_006409
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 56 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Olfactomedin-4, His Tag (Catalog # OL4-H5221) SDS-PAGE gel

Human Olfactomedin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Olfactomedin-4 (OLM-4) is also known as OLFM4, antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein (hGC-1), hOLfD, is a secreted protein which contains one olfactomedin-like domain. OLFM4 is expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. OLFM4 may promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. OLFM4 may play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. The human OLFM4 is also thought to be a useful marker for early myeloid development.

References

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