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Human GFR alpha-2 Protein  pdf  pdf  pdf


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GF2-H5225-200ug
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Synonym

GFRA2,GDNFRB,NRTNR-ALPHA,NTNRA,RETL2,TRNR2

Source

Human GFR alpha-2, His Tag (GF2-H5225) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 441 (Accession # NP_001486.4).

Predicted N-terminus: Ser 22

Molecular Characterization

GFR alpha-2(Ser 22 - Ser 441)NP_001486.4
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.6 kDa. The protein migrates as 60-70 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human GFR alpha-2, His Tag (Cat. No. GF2-H5225) SDS-PAGE gel

Human GFR alpha-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

 

Background

GDNF family receptor alpha-2 (GFRA2) is also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF-beta superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The isoform 1 of GFRA2 is found in both brain and placenta. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor and also able to mediate GDNF signaling through the RET tyrosine kinase receptor. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It can also mediate GDNF signaling through the RET tyrosine kinase receptor.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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