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Human IL-17E / IL-25 Protein  pdf  pdf  pdf


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Synonym

IL-17E,IL-25,Interleukin-25

Source

Human IL-17E, His Tag (IL5-H4221) is expressed from human 293 cells (HEK293). It contains AA Tyr 33 - Gly 177 (Accession # NP_073626.1).

Predicted N-terminus: Tyr 33

Molecular Characterization

IL-17E(Tyr 33 - Gly 177)NP_073626.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.7 kDa. The protein migrates as 21 kDa, 24 kDa and 26 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL-17E, His Tag (Cat. No. IL5-H4221) SDS-PAGE gel

Human IL-17E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Interleukin 25 (IL25) is also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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