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Your Position: Home > Enzymes > Human ADAM12 Protein

Human ADAM12 Protein

  • Synonym
    ADAM12,MCMP,MLTN,MLTNA,MCMPMltna,Meltrin Alpha
  • Source
    Human ADAM12, His Tag (AD2-H5228) is expressed from human 293 cells (HEK293). It contains AA Arg 29 - Asp 513 (Accession # AAH60804.1).
    Predicted N-terminus: Arg 29
  • Molecular Characterization
    ADAM12(Arg 29 - Asp 513) AAH60804.1

    rh ADAM12 is fused with 6 ×His tag at the C terminus, and has a calculated MW of 55.2 kDa (Arg 29 - Asp 513, Propeptide 20.1 kDa + mature form 34.5 kDa). The rh ADAM12 (Arg 29 - Asp 513) was cleaved into the propeptide and the mature form. The reducing (R) protein migrates as 65 kDa, 46 kDa and 23 kDa in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ADAM12, His Tag (Cat. No. AD2-H5228) SDS-PAGE gel

Human ADAM12, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-alpha and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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