Our mail server is under upgrade and may miss some of your emails. Please call +1 800-810-0816 to confirm when you get bounce notification or no responses from us.

Browse Products By Molecule:

Products --> MABSol® Fc Receptors

Human FcRn / FCGRT & B2M Heterodimer Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
FCM-H5286-50ug
    $350.00
FCM-H5286-1mg (500ug x 2)
    $2400.00
In-stock
Place Purchase Orders


Synonym

FcRn,FCGRT & B2M

Source

Human FcRn / FCGRT & B2M heterodimer protein, Ala 24 - Ser 297 (FCGRT, NP_001129491.1) and & Ile 21 - Met 119 (B2M, AAH32589), was produced in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

Molecular Characterization

FCGRT(Ala 24 - Ser 297)NP_001129491.1
Poly-his
B2M(Ile 21 - Met 119)AAH32589
Strep II

This protein contains two subunits. Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus.

The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human FcRn / FCGRT & B2M (Catalog # FCM-H5286) SDS-PAGE gel

Human FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Human FcRn / FCGRT & B2M (Catalog # FCM-H5286) ELISA bioactivity

Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Human FcRn / FCGRT & B2M (Cat# FCM-H5286 ) with a linear range of 0.06-5 μg/mL.

Request for protocol

Human FcRn / FCGRT & B2M (Catalog # FCM-H5286) ELISA bioactivity

Immobilized Human FcRn / FCGRT & B2M Heterodimer Protein (Cat# FCM-H5286) can bind human IgG1 with an affinity constant of 1 μM as determined in a SPR assay (Biacore 2000). The data is generally provided by Biaffin GmbH &Co KG, Germany.

 

Citations

Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.