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Your Position: Home > Cytokines & Growth Factors > Mouse KITLG /SCF Protein

Mouse SCF / KITLG Protein

  • Synonym
    KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL
  • Source
    Mouse SCF, His Tag (SCF-M5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Ala 189 (Accession # NP_038626).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    SCF(Lys 26 - Ala 189) NP_038626

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse SCF, His Tag (Cat. No. SCF-M5228) SDS-PAGE gel

Mouse SCF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse SCF, His TagMouse SCF, His Tag (Cat. No. SCF-M5228) ELISA bioactivity

Immobilized Human CD117, Fc Tag (Cat. No. CD7-H5255) at 2 μg/mL (100 μl/well) can bind Mouse SCF, His Tag (Cat. No. SCF-M5228) with a linear range of 0.05-1.2 ng/mL (QC tested).

  • Background
    Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $380.00

Inventory : In-stock

Price(USD) : $198.00

Inventory : In-stock

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