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Mouse SCF / KITLG Protein  pdf  pdf  pdf


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Synonym

KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

Source

Mouse SCF / KITLG Protein (Mouse SCF, His Tag) Lys 26 - Ala 189 (Accession # NP_038626) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

SCF (Lys 26-Ala 189)NP_038626

polyhistidine

Mouse SCF, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 19.1 kDa. The predicted N-terminus is Lys 26. The reducing (R) protein migrates as 19-30 kDa in SDS-PAGE due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Mouse SCF / KITLG Protein

Mouse SCF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity

(1) Immobilized Human CD117, Fc Tag (Cat# CD7-H5255) at 2 μg/mL (100 μl/well) can bind Mouse SCF, His Tag (Cat# SCF-M5228) with a linear range of 0.05-1.2 ng/mL.
(2) Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/mL.

 
 

Background

Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

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References