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Human Fc gamma RIIB / CD32b Protein  pdf  pdf  pdf


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CDB-H5228-100ug
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Synonym

FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32

Source

Human Fc gamma RIIB, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994).

Predicted N-terminus: Ala 46

Molecular Characterization

CD32b(Ala 46 - Pro 217)P31994
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Fc gamma RIIB, His Tag (Catalog # CDB-H5228) SDS-PAGE gel

Human Fc gamma RIIB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity


The bioactivity is measured by its binding ability to trastuzumab in a SPR assay. Immobilized Human Fc gamma RIIB / CD32b Protein, His tag (Cat# CDB-H5228) can bind trastuzumab with affinity constant of around 12.4 uM (routinely tested).

 

Background

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.