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Your Position: Home > Protein > TRAIL R2 > TR2-H5255

Human TRAIL R2 / DR5 / TNFRSF10B Protein, Fc Tag

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  • Synonym
    TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5,KILLER,TRICK2,ZTNFR9,TRICKB
  • Source
    Human TRAIL R2, Fc Tag(TR2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ile 56 - Glu 182 (Accession # NP_003833).
    Predicted N-terminus: Ile 56
  • Molecular Characterization
    TRAIL R2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 40.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TRAIL R2 SDS-PAGE

Human TRAIL R2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 TRAIL R2 ELISA

Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) with a linear range of 0.6-20 ng/mL (QC tested).

Bioactivity-BLI
 TRAIL R2 BLI

Loaded Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 0.146 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.
  • Clinical and Translational Updates

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Price(USD) : $260.00

Price(USD) : $1760.00

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  • Latest Research Phase:Approved

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