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Your Position: Home > Immune Checkpoint Proteins > Human PD-L1 B7-H1 Protein, With C-Fc Tag

Human PD-L1 / B7-H1 Protein, Fc Tag, low endotoxin

  • Synonym
    PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
  • Source
    Human PD-L1, Fc Tag (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    PD-L1(Phe 19 - Arg 238) NP_054862.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PD-L1, Fc Tag (Cat. No. PD1-H5258) SDS-PAGE gel

Human PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human PD-L1, Fc Tag (Cat. No. ) HPLC images

The purity of Human PD-L1, Fc Tag (HPLC-verified)(Cat.# PD1-H5258) was greater than 95% as determined by SEC-HPLC.

Bioactivity
Human PD-L1, Fc TagHuman PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 5 μg/mL ( 100 μl/well ) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 1-50 ng/mL (Routinely tested).

Human PD-L1, Fc TagHuman PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.31-1.25 μg/mL (QC tested).

Human FcRn Heterodimer Protein Cell_Base

Flow Cytometry assay shows that recombinant human PD-L1 (Cat. No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 μg/mL (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

FACS analysis shows that the binding of PD-L1 to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-hPD-L1 antibody. The concentration of PD-L1 used is 10 μg/mL. The IC50 is 12.92 μg/mL (Routinely tested).

  • Background
    Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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