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ActiveMax® Recombinant Human IFN-gamma /IFNG  pdf  pdf  pdf


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IFG-H4211-100ug
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Synonym

Interferon-gamma ,Interferon-γ

Source

ActiveMax® Human IFN-gamma (IFG-H4211) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # AAH70256).

Predicted N-terminus: Gln 24

Molecular Characterization

IFN-gamma(Gln 24 - Gln 166)AAH70256

This protein carries no "tag".

The protein has a calculated MW of 16.8 kDa. The protein migrates as 17 kDa,20 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human IFN-gamma (Cat. No. IFG-H4211) SDS-PAGE gel

ActiveMax® Human IFN-gamma on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

ActiveMax® Human IFN-gamma (Cat. No. IFG-H4211) ELISA bioactivity

The bio-activity of IFN-gamma(Cat. No. IFG-H4211)was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL (Routinely tested).

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Background

Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.