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MabAffinity rProtein A Prepacked Column  pdf  pdf  pdf

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MabAffinity rProtein A Agarose High Flow Resin is a classic protein A affinity medium for fast and efficient purification of monoclonal and polyclonal antibodies at any scale. The high specificity of rProtein A ligand to Fc domain of IgGs gives a high purification factor in a single chromatography step. Superior coupling chemistry ensures both high capacity and low leakage.


  1. Classic rProtein A resin suitable for different applications, including purification of poly or monoclonal antibodies of different species and subclasses and isolation of immune complex
  2. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity


Composition Highly cross-linked Agarose
Average particle size 90 micron
Form 5ml prepacked column;
Ligand rProtein A
Coupling chemistry Epoxy
Dynamic binding capacity1 > 150mg IgG /5ml prepacked column
Recommended flow rate 1-5ml/min for 5ml prepacked column
Column dimension 1.6x2.5 cm for 5ml prepacked column
Maximum Pressure Drop 0.3Mpa
Chemical stability Stable in all aqueous buffers commonly used in protein A chromatography
pH working range 3-10
pH CIP range (short term) 2-11
CIP stability 8M Urea, 6M Gua-HCl or 10mM NaOH+1M NaCl, 0.1M acetic acid
Temperature stability2 2-40oC
Storage 20% Ethanol
Shelf life 5 years
  1. Determined at 10% breakthrough at 4min residence time
  2. Delivered at room temperature, and recommended long-term storage at 2-8 oC

MabAffinity rProtein A Agarose High Flow Resin belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity rProtein A Agarose High Flow are also commonly used to purify proteins or protein complexes.

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