Our mail server is under upgrade and may miss some of your emails. Please call +1 800-810-0816 to confirm when you get bounce notification or no responses from us.

Browse Products By Molecule:

Products --> MabAffinity Chromatography Media

MabAffinity Protein L Prepacked Column  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
ML-0422-C5-5ml
    $395.00
In-Stock
Place Purchase Orders


MabAffinity Protein L Agarose High Flow Resin, with rProtein L coupled to high flow Agarose matrix, has been successfully used for a wide range of antibody and antibody fragments capture for its strong affinity to the variable region of antibody kappa light chains. Although it binds to the Fab portion of the immunoglobulin, Protein L does not interfere with the antigen-binding site of the antibody. Therefore, Protein L potentially can be used in immunoprecipitation (IP) procedures. As a good complementary choice for Protein A and Protein G, besides IgG, Protein L has been used for efficient purification of IgM, IgE, IgD, IgA and IgY. MabAffinity Protein L Agarose High Flow Resin also shows great advantages in purification of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Features:

  1. Binding specificities that complement Protein A and G media
  2. Suitable for different applications, including poly or monoclonal antibodies and antibody fragments from different species and subclasses for its broad binding specificity
  3. Purification of monoclonal antibodies from FCS supplemented media without interference of bovine immunoglobulin
  4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification:

Composition Highly cross-linked Agarose
Average particle size 90 micron
Form 5ml prepacked column
Ligand rProtein L
Coupling chemistry Epoxy
Dynamic binding capacity1 > 25‐50mg human IgG /5ml prepacked column
Recommended flow rate 1‐5ml/min for 5ml prepacked column
Column dimension 1.6x2.5 cm for 5ml prepacked column
Maximum Pressure Drop 0.3Mpa
Chemical stability Stable in all aqueous buffers commonly used in protein L chromatography
pH working range 2-10
pH CIP range (short term) 2-11
CIP stability 8M Urea, 10mM NaOH + 1M NaCl, 0.1M acetic acid
Temperature stability1 2-40oC
Storage 20% Ethanol
Shelf life 5 years
  1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

MabAffinity Protein L Agarose High Flow Resin belongs to the MabAffinity resins family for capture of antibodies and fragments containing kappa light chains, and is available with different pack sizes for easy purification, process development and scale up. Immunoprecipitation studies with MabAffinity Protein L Agarose High Flow are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

Related Products

Cat No Product Description Size/ Price
MA-0422-C5 MabAffinity rProtein A Prepacked Column 5ml 5ml ($290)
MA-0422-02 MabAffinity rProtein A Agarose High Flow 25ml ($635)
MA-0422-03 MabAffinity rProtein A Agarose High Flow 100ml ($1200)
MG-0422-C5 MabAffinity Protein G Prepacked Column 5ml 5ml ($350)
MG-0422-02 MabAffinity Protein G Agarose High Flow 25ml ($900)
MG-0422-03 MabAffinity Protein G Agarose High Flow 100ml ($1500)
ML-0422-C5 MabAffinity Protein L Prepacked Column 5ml 5ml ($395)
ML-0422-01 MabAffinity Protein L Agarose High Flow 5ml ($390)
ML-0422-02 MabAffinity Protein L Agarose High Flow 25ml ($1400)
ML-0422-03 MabAffinity Protein L Agarose High Flow 100ml ($2900)
FK-0422-C1 FabAffinity KBP Prepacked Column 1ml 1ml ($220)
FK-0422-C5 FabAffinity KBP Prepacked Column 5ml 5ml ($650)
FK-0422-01 FabAffinity KBP Agarose High Flow 5ml ($550)