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Human Osteopontin (OPN) Protein  pdf  pdf  pdf


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Synonym

SPP1,BNSP,OPN,Uropontin,Nephropontin,Osteopontin,BSP-1,ETA-1,BSPI

Source

Human Osteopontin (OPN) Protein (Human Osteopontin, His Tag) Ile 17 - Asn 300 (Accession # AAH07016) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Osteopontin, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 33 kDa. The predicted N-terminus is Ile 17. DTT-reduced Protein migrates as 60-65 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human Osteopontin, His Tag by the LAL method.

Purity

>80% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human Osteopontin (OPN) Protein
The purity of Human Osteopontin, His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Osteopontin (OPN) is also known as Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1, Nephropontin, Urinary stone protein, Uropontin, BNSP, which belongs to the osteopontin family. OPN / SPP1 is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. Full length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of the protein known as OPN-R. Osteopontin / SPP-1 is biosynthesized by a variety of tissue types. OPN is the ligand for integrin alpha-V/beta-3. OPN / SPP1 binds tightly to hydroxyapatite and appears to form an integral part of the mineralized matrix. OPN / SPP1 probably important to cell-matrix interaction. OPN / SPP1 acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

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References

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