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ActiveMax® Recombinant Human LIF  pdf  pdf  pdf


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LIF-H521b-50ug
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LIF-H521b-1mg
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LIF-H521b-20ug
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Synonym

LIF,CDF,DIA,HILDA,MLPLI

Source

ActiveMax® Human LIF (LIF-H521b) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1).

Predicted N-terminus: Ser 23

Molecular Characterization

LIF(Ser 23 - Phe 202)AAH69540.1

This protein carries no "tag".

The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human LIF (Catalog # LIF-H521b) SDS-PAGE gel

ActiveMax® Human LIF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

ActiveMax® Human LIF (Catalog # LIF-H521b) ELISA bioactivity

Immobilized Human LIF, Tag Free (Catalog # LIF-H521b) at 0.2μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Catalog # LIR-H4252) with a linear range of 7.8-31.25 ng/mL.

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Background

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.