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Influenza A Hemagglutinin (HA) Protein, A/Anhui/1/2013(H7N9)  pdf


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HA9-V5229-100ug
HA9-V5229-1mg
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Synonym

HA, Hemagglutinin, H7N9

Source

Influenza A Hemagglutinin (HA) Protein, A/Anhui/1/2013(H7N9) (HA(H7N9)-Anhui) Asp 19 - Asp 521 was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

HA(H7N9)-Anhui is fused with 6×His tag at the C-terminus, and has a calculated MW of 58.6 kDa. The predicted N-terminus is Asp 19. DTT-reduced Protein migrates as 63-75 kDa.

Endotoxin

Less than 1.0 EU per μg of the HA(H7N9)-Shanghai by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

 

SDS-PAGE


Influenza A Hemagglutinin (HA) Protein,  A/Anhui/1/2013(H7N9)
The purity of Influenza A Hemagglutinin (HA) Protein, A/Anhui/1/2013(H7N9) was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue.

Bioactivity


Measured by its binding ability in a functional ELISA. Immobilized Influenza HA,c-His Protein (HA9-V5229) at 2 μg/ml can bind Anti-Influenza HA MAb CM2S3 (CR1-M2S3) with a linear range of 0.5–20 μg/ml when use a secondary antibody HRP*-Goat anti Human IgG, Fcγ Fragment Specific,1:5000 from Jackson.

 

Background

Influenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.

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References