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Biotinylated Human Growth Hormone R, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

GHR,GHBP,GH receptor

Source

MABSol® Biotinylated Human Growth Hormone R (GHR) (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912). It is the biotinylated form of Human Growth Hormone R (GHR) Protein (Cat # GHR-H5222).

Predicted N-terminus: Ala 27

Molecular Characterization

GHR(Ala 27 - Tyr 264)P10912
Poly-his

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 1-3 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human Growth Hormone R (Cat. No. GHR-H8222) SDS-PAGE gel

Biotinylated Human Growth Hormone R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Human Growth Hormone R (Cat. No. GHR-H8222) ELISA bioactivity

Immobilized Human GH, Tag Free (Cat. No. GH1-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GHR, His Tag (Cat. No. GHR-H8222) with a linear range of 2-31 ng/mL (QC tested).

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Background

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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