Human CD48, His Tag (BC1-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # AAH16182).
Predicted N-terminus: Gln 27
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.2 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human 2B4, Fc Tag (Catalog # 2B4-H5255) at 5 μg/mL (100 μL/well) can bind Human CD48, His Tag (Catalog # BC1-H5226) with a linear range of 0.156-2.5 μg/mL.
CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.
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