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Human CD48 / SLAMF2 Protein  pdf  pdf  pdf


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Synonym

CD48,BCM1,SLAMF2,BLAST1,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1

Source

Human CD48, His Tag (BC1-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # AAH16182).

Predicted N-terminus: Gln 27

Molecular Characterization

CD48(Gln 27 - Ser 220)AAH16182
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.2 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD48, His Tag (Catalog # BC1-H5226) SDS-PAGE gel

Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human CD48, His Tag (Catalog # BC1-H5226) ELISA bioactivity

Immobilized Human 2B4, Fc Tag (Catalog # 2B4-H5255) at 5 μg/mL (100 μL/well) can bind Human CD48, His Tag (Catalog # BC1-H5226) with a linear range of 0.156-2.5 μg/mL.

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Background

CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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