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Mouse Anti c-Myc Tag Monoclonal Antibody (9E10)  pdf  pdf  pdf


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MYC-MMYCc-100ug
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Basic Information

Species Reactivity Mouse
Host Cells HEK293 cells
MW 50 and 25 kDa for the heavy and light chains, respectively (150 kDa)
Isotype Mouse IgG1, Kappa LC
Clonality Monoclonal

Synonym

c-Myc, MYC, MRTL, bHLHe39

Applications

Western Blotting (0.5-2 μg/ml)
ELISA (0.2-1 μg/ml)
Immunoprecipitation (2-5 μg/IP)
Immunofluorescence (2-10 μ g/ml)
Widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc (amino acids 408-439) epitope tag

*Optimal dilutions should be determined by the researcher.

Immunogen

Epitope corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSC) within the carboxy terminal domain of c-Myc of human origin

Specificity

This Mab reacts specifically to the c-myc epitope EQKLISEEDL. C-Myc Mab (9E10) can be used to detect c-Myc tagged proteins and the native protein derived from the c-myc proto-oncogene

Purification

Protein A purified from cell culture supernatant

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0 with trehalose as protectant before lyophilization.
Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Lyophilized antibody should be stored at -20oC or lower for long term storage.Upon reconstitution,working aliquots should be stored at -20oC to -70oC.

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC- 8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 12 months (-20oC to -70oC).

Background

c-Myc is also known as MYC, which belongs to Myc family of transcription factors, which also includes N-Myc and L-Myc. Myc family of transcription factors contain bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain. Myc protein, through its bHLH domain can bind to DNA, while the leucine zipper domain allows the dimerization with its partner Max, another bHLH transcription factor. A mutated version of Myc is found in many cancers, which causes Myc to be constitutively (persistently) expressed. This leads to the unregulated expression of many genes, some of which are involved in cell proliferation and results in the formation of cancer. A common human translocation involving Myc is t(8;14) which is critical to the development of most cases of Burkitt's Lymphoma. c-Myc induces AEG-1 or MTDH gene expression and in turn itself requires AEG-1 oncogene for its expression. c-Myc is associated with carcinoma of the cervix, colon, breast, lung and stomach.c-Myc participates in the regulation of gene transcription, binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. c-Myc seems to activate the transcription of growth-related genes.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References

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