Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Enzymes

Human Kallikrein 11 / KLK-11 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
KL1-H5224-20ug
    $290.00
KL1-H5224-1mg (500ug x 2)
    $2000.00
In-stock
Place Purchase Orders


Synonym

KLK11,Kallikrein-11,Hippostasin,hK11,PRSS20,TLSP

Source

Human Kallikrein 11, His Tag (KL1-H5224) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 250 (Accession # AAH22068).

Predicted N-terminus: Glu 19

Molecular Characterization

Kallikrein 11(Glu 19 - Asn 250)AAH22068
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Kallikrein 11, His Tag (Cat. No. KL1-H5224) SDS-PAGE gel

Human Kallikrein 11, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK11 contains one peptidase S1 domain. KLK11 is expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine but not lysine residues.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Related Products

Questions & Comments