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Human Kallikrein 11 / KLK-11 Protein  pdf  pdf  pdf


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Synonym

KLK11, Kallikrein-11, Hippostasin, hK11, PRSS20, TLSP

Source

Recombinant Human KLK11 /Kallikrein-11 Protein (rhKallikrein-11 /KLK11) Glu 19 - Asn 250 (Accession # AAH22068) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

rhKallikrein-11 /KLK11, fused with 6×His tag at the C-terminus, has a calculated MW of 26.5 kDa. The predicted N-terminus is Glu 19. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rhKallikrein-11 /KLK11 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human KLK11 /Kallikrein-11 Protein
The purity of rhKallikrein-11 /KLK11 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK11 contains one peptidase S1 domain. KLK11 is expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine but not lysine residues.

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References

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