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Human Kallikrein 4 / KLK-4 Protein  pdf  pdf  pdf


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KL4-H5227-50ug
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Synonym

KLK4,Kallikrein-4,Prostase,KLK-L1,EMSP1,PRSS17,PSTS

Source

Human Kallikrein 4, His Tag (KL4-H5227) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Ser 254 (Accession # AAH69429).

Predicted N-terminus: Ser 27

Molecular Characterization

Kallikrein 4(Ser 27 - Ser 254)AAH69429
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.2 kDa. The protein migrates as 30-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Kallikrein 4, His Tag (Catalog # KL4-H5227) SDS-PAGE gel

Human Kallikrein 4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Kallikrein-4 (KLK4) is also known as Enamel matrix serine proteinase 1 (EMSP1), Kallikrein-like protein 1 (KLK-L1), Prostase, Serine protease 17 (PRSS17), PSTS, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK4 contains one peptidase S1 domain. KLK4 is expressed in prostate and involved in enamel formation. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

References

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