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Human IgG4 Fc Protein, Tag Free  pdf  pdf  pdf


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Synonym

IgG4 Fc, IGHG4

Source

Recombinant Human IgG4 Fc Region Protein (Human IgG4 Fc Region) Glu 99 - Lys 327 (Accession # P01861) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

IgG4 Fc(Glu 99-Lys 327)P01861

Human IgG4 Fc, Tag Free contains no "tag", and has a calculated MW of 25.8 kDa. The predicted N-terminus is Glu 99. As a result of glycosylation, the reducing (R) protein migrates as 33-35 kDa, and the non-reducing (NR) protein migrates as 50-55 kDa in SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE

Recombinant Human IgG4 Fc Region Protein

Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 
 

Background

Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References