IgG4 Fc, IGHG4
Recombinant Human IgG4 Fc Region Protein (Human IgG4 Fc Region) Glu 99 - Lys 327 (Accession # P01861) was produced in human 293 cells (HEK293) at ACRObiosystems.
Human IgG4 Fc, Tag Free contains no "tag", and has a calculated MW of 25.8 kDa. The predicted N-terminus is Glu 99. As a result of glycosylation, the reducing (R) protein migrates as 33-35 kDa, and the non-reducing (NR) protein migrates as 50-55 kDa in SDS-PAGE.
Less than 1.0 EU per μg the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
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