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Your Position: Home > Other Recombinant Proteins > Human IgG2 Fc Protein

Human IgG2 Fc Protein, Tag Free

  • Synonym
    IgG Fc
  • Source
    Human IgG2 Fc, Tag Free (IG2-H5206) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 326 (Accession # P01859).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    IgG Fc(Glu 99 - Lys 326) P01859

    This protein carries no "tag".

    The protein has a calculated MW of 25.7 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206) SDS-PAGE gel

Human IgG2 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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