Human Cystatin D, His Tag (CS5-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Val 142 (Accession # AAH69514).
Predicted N-terminus: Gly 21
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.7 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Cystatin D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Cystatin-5 (CST5) is also known as Cystatin-D, is a secreted thermostable protein, which belongs to the cystatin family. CST5 is a cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. CST5 / cystatin-D may play a protective role against proteinases present in the oral cavity.
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