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Human Kallikrein 3 / KLK-3 Protein  pdf  pdf  pdf


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Synonym

KLK3,Kallikrein-3,APS,KLK2A1,PSA,Hk3,Seminin,Semenogelase

Source

Human Kallikrein 3 / KLK-3 Protein (Human Kallikrein 3, His Tag) Ala 18 - Pro 261 (Accession # NP_001639) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Kallikrein 3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. The reducing (R) protein migrates as 36 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human Kallikrein 3 / KLK-3 Protein
Human Kallikrein 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
 
 

Background

Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

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References

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