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Human Kallikrein 3 / KLK-3 Protein  pdf  pdf  pdf


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KL3-H5226-50ug
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Synonym

KLK3,Kallikrein-3,APS,KLK2A1,PSA,Hk3,Seminin,Semenogelase

Source

Human Kallikrein 3, His Tag (KL3-H5226) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Pro 261 (Accession # NP_001639).

Predicted N-terminus: Ala 18

Molecular Characterization

Kallikrein 3(Ala 18 - Pro 261)NP_001639
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.7 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Kallikrein 3, His Tag (Cat. No. KL3-H5226) SDS-PAGE gel

Human Kallikrein 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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