Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Bio-Markers & CD Antigens

Human Mesothelin / MSLN Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
MSN-H5223-100ug
    $315.00
MSN-H5223-1mg (500ug x 2)
    $1980.00
In-stock
Place Purchase Orders


Synonym

MSLN,Mesothelin,MPF

Source

Human Mesothelin (aa 296-580), His Tag (MSN-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).

Predicted N-terminus: Glu 296

Molecular Characterization

Mesothelin(Glu 296 - Gly 580)AAH09272
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33 kDa. The protein migrates as 38-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Mesothelin (aa 296-580), His Tag (Cat. No. MSN-H5223) SDS-PAGE gel

Human Mesothelin (aa 296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human Mesothelin (aa 296-580), His Tag (Cat. No. MSN-H5223) ELISA bioactivity

Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 2 μg/mL (100 μL/well) can bind Anti-MSLN MAb, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

Request for protocol

 

Background

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Related Products

Questions & Comments