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Human CD27 Ligand / CD70 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

Source

Human CD27 Ligand, Fc Tag (TN7-H526x) is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # AAH00725).

Predicted N-terminus: Glu

Molecular Characterization

Fc(Thr 106 - Lys 330)P01857
CD27 Ligand(Gln 39 - Pro 193)AAH00725

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 44.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD27 Ligand, Fc Tag (Catalog # TN7-H526x) SDS-PAGE gel

Human CD27 Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

Human CD27 Ligand, Fc Tag (Catalog # TN7-H526x) ELISA bioactivity

Immobilized Human CD27 Ligand, Fc Tag (Catalog # TN7-H526x) at 10μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc Tag (Catalog # TN7-H82F6) with a linear range of 4.9-156 ng/mL.

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Background

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

References

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