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Human Kallikrein 1 / KLK-1 Protein  pdf  pdf  pdf


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Synonym

KLK1, Kallikrein-1, KLKR, Klk6, hK1

Source

Recombinant Human KLK1 /Kallikrein-1 Protein (rhKallikrein-1 /KLK1) Pro 19 - Ser 262 (Accession # NP_002248) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

rhKallikrein-1 /KLK1, fused with polyhistidine tag at the C-terminus, has a calculated MW of 27.9 kDa. The predicted N-terminus is Pro 19. DTT-reduced Protein migrates as 40-45 kDa due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rhKallikrein-1 /KLK1 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human KLK1 /Kallikrein-1 Protein
The purity of rhKallikrein-1 /KLK1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

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References