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Human Kallikrein 1 / KLK-1 Protein  pdf  pdf  pdf


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KL1-H5223-50ug
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Synonym

KLK1,Kallikrein-1,KLKR,Klk6,hK1

Source

Human Kallikrein 1, His Tag (KL1-H5223) is expressed from human 293 cells (HEK293). It contains AA Pro 19 - Ser 262 (Accession # NP_002248).

Predicted N-terminus: Pro 19

Molecular Characterization

Kallikrein 1(Pro 19 - Ser 262)NP_002248
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.9 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Kallikrein 1, His Tag (Cat. No. KL1-H5223) SDS-PAGE gel

Human Kallikrein 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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