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Recombinant Human Cystatin F / CST7 Protein  pdf  pdf  pdf


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CS7-H5222-50ug
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CS7-H5222-1mg
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Synonym

CST7,Cystatin-F,Cystatin-7,CMAP,Leukocystatin,Cystatin 7

Source

Human Cystatin F, His Tag (CS7-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 42 - His 167 (Accession # AAH15507).

Predicted N-terminus: Gly 42

Molecular Characterization

Cystatin F(Gly 42 - His 167)AAH15507
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.6 kDa. The protein migrates as 20-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Cystatin F, His Tag (Cat. No. CS7-H5222) SDS-PAGE gel

Human Cystatin F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Cystatin-7 (CST7) is also known as Cystatin-F, Cystatin-like metastasis-associated protein (CMAP), Leukocystatin, which is a disulfide-linked homodimer belongs to the cystatin family. CST7 is primarily expressed in peripheral blood cells and spleen. CST7 inhibits papain and cathepsin L but with affinities lower than other cystatins. CST7 may play a role in immune regulation through inhibition of a unique target in the hematopoietic system.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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