Human Myocilin, His Tag (MYC-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 33 - Met 504 (Accession # NP_000252).
Predicted N-terminus: Glu 214
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE) due to most of the MYOC was cleaved at Glu 214, generating a C-terminal fragment.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Myocilin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Myocilin, trabecular meshwork inducible glucocorticoid response is also known as MYOC, GLC1A, GPOA, JOAG, JOAG1, TIGR. Myocilin / MYOC contains one olfactomedin-like domain. Myocilin is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.
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