Human Carboxypeptidase A4, His Tag (CP4-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Tyr 421 (Accession # AAH52289).
Predicted N-terminus: Gly 17
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.4 kDa. The protein migrates as 48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Carboxypeptidase A4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. Carboxypeptidases cleave amino acids from the C-terminus of proteins and peptides and many are metalloproteases. Carboxypeptidase A4 (CPA4) is also known as Carboxypeptidase A3 (CPA3), which belongs to the peptidase M14 family. Fetal express the protein in the adrenal gland, brain, heart, intestine, kidney, liver and lung.
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