Browse Products By Molecule:

Products --> Enzymes

Human Carboxypeptidase A4 / CPA4 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
CP4-H5222-50ug
    $315.00
0
Place Purchase Orders


Synonym

CPA4, Carboxypeptidase A4, CPA3

Source

Recombinant Human Carboxypeptidase A4 /CPA4 Protein (rh CPA4 / Carboxypeptidase A4) Gly 17 - Tyr 421 (Accession # AAH52289) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

rh CPA4 / Carboxypeptidase A4, fused with polyhistidine tag at the C-terminus, has a calculated MW of 46.4 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 48 kDa due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh CPA4 / Carboxypeptidase A4 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human Carboxypeptidase A4 /CPA4 Protein
The purity of rh CPA4 / Carboxypeptidase A4 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 
 

Background

Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. Carboxypeptidases cleave amino acids from the C-terminus of proteins and peptides and many are metalloproteases. Carboxypeptidase A4 (CPA4) is also known as Carboxypeptidase A3 (CPA3), which belongs to the peptidase M14 family. Fetal express the protein in the adrenal gland, brain, heart, intestine, kidney, liver and lung.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References