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Human Carboxypeptidase A4 / CPA4 Protein  pdf  pdf  pdf


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CP4-H5222-50ug
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Synonym

CPA4,Carboxypeptidase A4,CPA3

Source

Human Carboxypeptidase A4, His Tag (CP4-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Tyr 421 (Accession # AAH52289).

Predicted N-terminus: Gly 17

Molecular Characterization

CPA4(Gly 17 - Tyr 421)AAH52289
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 46.4 kDa. The protein migrates as 48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Carboxypeptidase A4, His Tag (Cat. No. CP4-H5222) SDS-PAGE gel

Human Carboxypeptidase A4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. Carboxypeptidases cleave amino acids from the C-terminus of proteins and peptides and many are metalloproteases. Carboxypeptidase A4 (CPA4) is also known as Carboxypeptidase A3 (CPA3), which belongs to the peptidase M14 family. Fetal express the protein in the adrenal gland, brain, heart, intestine, kidney, liver and lung.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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