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Human VEGF-D Protein  pdf  pdf  pdf


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Synonym

FIGF,VEGFD

Source

Human VEGF-D, His Tag (VED-H5228) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).

Predicted N-terminus: Phe 93

Molecular Characterization

VEGF-D(Phe 93 - Ser 201)AAH27948
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 13 kDa. The protein migrates as 18-20 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human VEGF-D, His Tag (Catalog # VED-H5228) SDS-PAGE gel

Human VEGF-D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human VEGF-D, His Tag (Catalog # VED-H5228) ELISA bioactivity

Immobilized Human VEGF R3, Fc Tag (Catalog # FL4-H5251) at 5μg/mL (100 μL/well) can bind Human VEGF-D, His Tag, Strep Tag (Catalog # VED-H5228) with a linear range of 0.08-0.6 μg/mL.

 

Background

Vascular endothelial growth factor D (VEGF-D) is also known as C-fos induced growth factor (FIGF), which belongs to the PDGF / VEGF growth factor family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. This secreted protein VEGF-D / FIGF undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3. The structure and function of this protein is similar to those of VEGFC. FIGF / VEGF-D is highly expressed in lung, heart, small intestine and fetal lung. FIGF / VEGF-D may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR / FLK1) and VEGFR-3 (FLT4) receptors.

References

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