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Your Position: Home > Cytokines & Growth Factors > Human FIGF /VEGFD Protein,HEK293 expressed

Human VEGF-D Protein

  • Synonym
    FIGF,VEGFD
  • Source
    Human VEGF-D, His Tag (VED-H5228) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).
    Predicted N-terminus: Phe 93
  • Molecular Characterization
    VEGF-D(Phe 93 - Ser 201) AAH27948

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 13 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human VEGF-D, His Tag (Cat. No. VED-H5228) SDS-PAGE gel

Human VEGF-D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human VEGF-D, His TagHuman VEGF-D, His Tag (Cat. No. VED-H5228) ELISA bioactivity

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5μg/mL (100 μL/well) can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with a linear range of 0.08-0.6 μg/mL (QC tested).

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