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Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M Heterodimer Protein  pdf  pdf  pdf


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Synonym

FcRn,FCGRT & B2M

Source

Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M Heterodimer Protein, Ala 24 - Ser 297 (FCGRT, Q8SPV9-1) and Ile 21 - Met 119 (B2M, Q8SPW0-1), was produced in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization

FCGRT(Ala 24 - Ser 297)Q8SPV9-1
Poly-his
B2M(Ile 21 - Met 119)Q8SPWO-1
Strep II

This protein contains two subunits. Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus.

The protein has a calculated MW of 32.3 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 36 kDa (FCGRT), 14 kDa (B2M) respectively under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M (Cat. No. FCM-C5284) SDS-PAGE gel

Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M (Cat. No. FCM-C5284) ELISA bioactivity

Immobilized Yervoy Ipilimumab at 10 μg/mL (100 µl/well),can bind Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M (Cat. No. FCM-C5284) with a linear range of 1.25-10 μg/mL (QC tested).

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Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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